动物造模 z-bio 2021-02-21 285

　　Objective: To explore the optimal fixation and storage conditions of frozen tissue sections in the immunohistochemical staining experiment of phosphorylated human epidermal growth factor receptor 2 (human epidermal growth factor receptor 2, HER2).

　　Methods: How BALB/c-nu/nu established a subcutaneous transplantation tumor model of human breast cancer cell BT474 in mice, and observed the immunohistochemical staining signal of phosphorylated HER2 in the tumor model by comparing various antigen fixation conditions and storage time The change.

　　Result: The frozen sections were fixed in 10% formaldehyde solution for 5 minutes. This allows you to maintain good cell morphology. Under storage conditions of -20°C, the expression of phosphorylated HER2 protein in tumor tissues is stable within 12 weeks.

　　Conclusion: Frozen tissue sections require a short time for immunohistochemical staining, which can be used as a supplement or alternative to traditional paraffin section staining methods.