动物造模,胆管癌细胞自噬 z-bio 2021-11-06 363

　　OBJECTIVE: To observe the effect of lactate dehydrogenase A (LDHA) inhibitor sodium oxalate on autophagy and apoptosis of human cholangiocarcinoma cell line Hucct-1, and to preliminarily explore its related mechanisms.

　　Method: CCK8 method detects cell proliferation after treating Hucct-1 cells with various concentrations of oxalic acid or transfecting Hucct-1 cells with LDHA small interfering RNA, which is an economic site indicator for detecting apoptosis and scratch healing The test detects cell migration, real-time fluorescent quantitative PCR and Western blotting detect autophagy-related mRNA and protein expression levels, respectively, and the lactic acid detection kit detects intracellular lactic acid content.

　　Result: Sodium oxalate is dose-dependent on the proliferation of Hucct-1 cells (P\u003c0.01), half of which is 50 mmol/L. Intervention with 50 mmol/L sodium oxalate in Hucct-1 cells or LDHA small cells. After NA was transfected into interfering Hucct-1 cells, LDH amRN and protein expression levels decreased (both P\u003c0.01), intracellular lactic acid content decreased (P\u003c0.01), and the rate of apoptosis increased ( P\u003c0). 01), shortened cell migration distance (P\u003c0.01), intracellular autophagy-related gene 7 (autophagy-related gene 7, ATG7), beclin-1 and microtubule-related protein 1 light chain 3II (microtubule-related Protein 1 light chain 3II, LC-3II) increased (both P\u003c0.01), while the expression level of P62 protein decreased (P\u003c0.01).

　　Conclusion: Lanin sodium promotes autophagy and induces apoptosis by inhibiting the expression of LDHA in Hucct-1 cells.