动物实验 z-bio 2021-12-27 329

　　Objective: To study the effects of Okadaic acid (OA) on the neurobehavioral function of zebrafish larvae, and to explore the application prospects of zebrafish models in neurodegenerative diseases.

　　Method: Use 0.05, 0.10, 0.20μmol/L OA solution to process 3d (3dpf) zebrafish fry 7 days after fertilization, record fry mortality, and use behavior analysis system to collect fry swimming trajectory within 30 minutes. , Measure the swimming distance and stagnation time of each group of fry. Real-time fluorescent quantitative PCR was used to detect the expression of juvenile protein phosphatase 2A (PP2A) and α7 nicotinic acetylcholine receptor (α7-nAChR) in each treatment group. The survival rate of adolescents in the 0.05μmol/L group and 0.10μmol/LOA group after 7 days of treatment was similar to

　　 There was no significant difference compared with the control group (P\→0.05), and there was no change in swimming trajectory. Compared with the control group, the survival rate of fry in the 0.20μmol/LOA group was significantly reduced, swimming activities were significantly reduced, swimming distance was significantly shortened, and stagnation time was significantly prolonged. (All P values are 0.05)).

　　 Conclusion: Treatment with 0.20μmol/LOA for 7 days can cause abnormal neurobehavior of zebrafish juveniles.