动物造模,人肺癌细胞建立骨转移小鼠模型 z-bio 2021-10-14 375

　　OBJECTIVE: To establish a mouse model of bone metastasis by intracardiac injection of human lung cancer cells under ultrasound guidance. Methods: Under ultrasound guidance, human lung cancer A549 and NCl-H1299 cells were injected into the left ventricle of NOD/SCID and BALB/c-nu/nu mice, respectively, and combined with real-time imaging of small animals, X-rays and small animals ,I did it. Animal micro-CT uses various small animal diagnostic imaging methods to establish models and observe the development of bone metastases. Use HE staining method to observe the pathology of tumor tissue.

　　Result: All animals survived intracardiac injection of lung cancer cells under ultrasound guidance. In vivo imaging of small animals showed that the bones of the central axis of the mouse and the bones of the limbs all have light-emitting areas. X-rays and small animal Micro-CT performed three-dimensional reconstruction of the entire bones of mice, showing obvious bone and periosteum defects, damage to the tibia plateau and shoulder joints of the mice, and normal bone tissue morphology. Gone, showing osteolysis. These changes and their imaging findings are consistent with changes in tumor bone destruction. Gross anatomy showed that the axial bones and limbs of the mice were wrapped in tumor tissue, and there were different degrees of bone metastasis.

　　Conclusion: Ultrasound-guided intracardiac injection of lung cancer bone metastasis model can effectively improve the success rate of modeling, truly simulate clinical lung cancer bone metastasis and the biological characteristics of lung cancer metastasis patients, and can be used to study the mechanism. -Transfer therapy and individual therapy.