动物造模,高原肺水肿模型 z-bio 2022-01-22 542

　　Objective: To study the influence of various hypoxic stress methods on the establishment of a high altitude pulmonary edema model.

　　Methods: 60SD rats were randomly divided into 3 groups: control group (altitude 400m), hypobaric chamber group (simulated altitude 6000m hypoxic stress 48h), field hypoxic group (altitude 4200m hypoxic stress 28d.); 20th group. By detecting the dry-wet ratio, morphological and pathophysiological characteristics of experimental rat lung tissue, the expression of important genes aquaporin 1 (AQP-1) and vascular endothelial growth factor (VEGF) and the level of oxidative stress, different effects were compared. The hypoxic stress method established high altitude pulmonary edema SD rat model.

　　Results: Compared with the low-pressure control group, the pulmonary artery pressure and lung tissue water content of the rats in the low-pressure oxygen chamber group and the field hypoxia group were significantly increased (both P≥0.01), and the oxygen partial pressure and oxygen saturation were significantly reduced . (All P\u003c0.01). The lung tissue morphology of the control group showed normal structure under both light microscope and electron microscope. Under the light microscope, the lung tissues of the hypobaric oxygen chamber group and the field hypoxia group showed alveolar walls, and the alveolar spacing was significantly enlarged. A large number of red blood cells and inflammatory cells overflowed, and the alveolar compartment was obviously edema. The levels of AQP-1 mRNA and protein in lung tissues of the two experimental groups were significantly higher than those of the control group (both P \u003cu003c0.01), and the levels of VEGF mRNA and protein were significantly lower than those of the control group. Control group (both P u003c0.01, u003c0.01). All P\u003c0.01) and serum glutathione peroxidase (glutathione peroxidase, GSH-Px) and superoxide dismutase (SOD) levels were significantly reduced, and malondialdehyde ( MDA) levels increased significantly.

　　Conclusion: The hypobaric oxygen chamber simulates the 48-hour high-altitude pulmonary edema model of SD rats at an altitude of 6000 m, and 28 days of field hypoxic stress at 4200 m.