动物造模,损伤性卵巢早衰模型 z-bio 2021-02-22 355

　　OBJECTIVE: To explore the method of establishing chemotherapy-induced primary ovarian insufficiency (POF) rat model by intraperitoneal injection of different doses of cisplatin (CP).

　　Method: Take 48 female Wisar fetal rats and divide them into 6 groups with 8 rats in each group. Different doses of CP were injected intraperitoneally at different times. The control group (group A) was untreated; CP intraperitoneal low-dose (1.5 mg/kg) injection group 1 (group B), low-dose (2.0 mg kg) injection group 2 (group C) and low-dose (2.5 mg/kg) injection group kg) group 3 (group D), 5 consecutive days a day; high-dose group 1 (group E) CP injected intraperitoneally, 4 mg/kg weekly, 4 mg/kg weekly re-injection; high-dose CP intraperitoneal injection- In the 2F group, 6 mg/kg and 6 mg/kg were injected every 4 weeks to establish a POF model induced by chemotherapy in rats. Observe the general condition of the rat, the estrus cycle, and detect changes in body weight and serum anti-Müllerian hormone (AMH) levels, as well as pathological changes in the number of ovaries and follicles at all levels. Compared with group A, the estrus cycle of rats in each treatment group was obviously disordered, the weight was significantly reduced, and the serum AMH level was also significantly reduced (P\u003c0.05). The decrease in E was the most obvious; each model group had different ovaries, E The number of follicles in group F and F was significantly reduced (P\u003c0.05), and the number of follicles at each level was significantly reduced.

　　Conclusion: In rats, intraperitoneal injection of 4 mg/kg CP and weekly reinjection has a better effect on modeling chemotherapy-induced POF, and the modeling is simple.