动物造模,非酒精性脂肪肝病食蟹猴模型 z-bio 2021-02-22 296

　　Objective: To establish an animal model of non-alcoholic fatty liver disease induced by high-fat diet, analyze the effect of high-fat feed feeding time on the formation of animal NAFLD, and study the high-fat feed feeding time. Serum biochemical indicators are related to the formation of fatty liver and fibrosis.

　　Method: Before the experiment, 700 healthy cynomolgus monkeys were drawn from the basal blood and fed a high-fat diet. When given for 1 year, 2 years, 3 years, 4 years, 5 years, 6 years and 7 years, a part of experimental monkeys were randomly selected, anesthetized with ketamine hydrochloride, and animal blood was collected. ), serum aspartate aminotransferase (AST), triglycerides (TG) and total cholesterol (TC)), high density lipoprotein (HDL), low density lipoprotein (LDL) indicators and liver biopsy.

　　Result: After feeding on a high-fat diet for 2 years, the serum ALT, AST, TG, TC and LDL levels of cynomolgus monkeys increased significantly, while the HDL level decreased significantly (P\u003c0.01). High-fat diet for more than 2 years was significantly correlated with ALT, AST, TG, TC, lipidation and fibrosis at the level of 0.01 (bilateral) (r is 0.127, 0.121, 0.246, 0.128, 0.306, respectively, 0.220). ), was negatively correlated with HDL (r = 0.298, P\u003c0.05), and negatively correlated with LDL (at the 0.05 level, the two were significantly correlated (r = -0.081).

　　 Conclusion: The blood of crab monkeys is rich in high-fat diet, which may significantly increase blood lipids and liver enzyme activity indicators, liver blood lipid accumulation and obvious blood lipid levels. Inflammatory invasion and high-fat diet can successfully induce NAFLD cynomolgus monkey model and promote NAFLD process.