动物造模,门脉高压动物模型 z-bio 2021-02-22 303

　　Objective: To investigate whether ligation of the left portal vein can form stable portal hypertension, and whether nitric oxide plays a corresponding role.

　　Method: 36 SPF male 8-week-old SD rats were randomly divided into a left branch ligation group (with needles with diameters of 0.6, 0.7 and 0.8 mm, respectively) and a main ligation group (with a diameter of 0.9 mm). Needle ligation is divided into 6 groups, left lateral lobectomy group and sham operation group. The portal pressure of all rats was measured before operation, after operation, 15 days after operation, and 30 days after operation. On the 30th day after the operation, all rats were sacrificed, and portal vein blood and left and right liver tissues were collected to measure the content of nitric oxide (NO).

　　Results: After the operation, the portal pressure of the left branch ligation group of SD rats was significantly higher than that of the preoperative and control groups at 15 and 30 days after the operation. There was no significant difference between the left branch ligation group. 0.6 mm group. The main 0.9 mm group was 30 days after operation. There was no statistical difference in NO content in the left and right liver tissues and blood of 6 SD rats.

　　Conclusion: Ligation of the left portal artery can form stable portal hypertension, and a needle with a diameter of 0.6 mm can achieve better results.