动物造模,咳嗽变异型哮喘豚鼠模型 z-bio 2023-01-30 545

　　Goal: Try to use ovalbumin in combination with smoking to establish a more similar guinea pig CVA model.

　　 Method: Randomly select 24 SPF male and female guinea pigs, divide them into 8 guinea pigs, a blank control group, an OVA sensitization group and an OVA sensitization + smoking group, a total of three groups. The OVA sensitization group was modeled by the ovalbumin sensitization method, and the OVA sensitization + smoking group was modeled by the ovalbumin sensitization method and the smoking method. The other methods are the same. Three groups of guinea pigs were tested for lung compliance (Cydn) after acetylcholine challenge, airway resistance (RI), number of coughs and time to first cough after capsaicin challenge, lung histopathology and Ashcroft scale score The completed model is evaluated for changes in the index.

　　 Results: Compared with the blank control group, the Cydn of the OVA sensitization group and the OVA sensitization + smoking group were significantly reduced, the RI was significantly increased, and the number of coughs increased significantly within 10 minutes after the first cough. Significantly faster, the pathological changes of lung tissue are obvious, and the score of Ashcroft classification table is significantly improved. The above performance is consistent with the characteristics of cough and asthma. At the same time, compared with the OVA sensitization group, the OVA sensitization + smoking group performed better in terms of cough symptoms and RI changes, which is more in line with the characteristics of cough atypical asthma. This may be related to the decrease in OVA. In order to avoid excessive immune response, the concentration of dense smoke is closely related to the increase in cough sensitivity.

　　 Conclusion: The combination of OVA sensitization method and smoking can establish a highly similar cough and asthma guinea pig model.