动物造模,冠心病气虚血瘀证病证结合大鼠模型 z-bio 2022-03-17 349

　　OBJECTIVE: Combining the pathology, etiology and tongue symptoms of the compound model of prostaglandin (PGI2) and thromboxane A2 (TXA2) in the rat model, to explore the relationship between coronary heart disease (CHD) deficiency and blood stasis syndrome. The model method of coronary heart disease with Qi deficiency syndrome and blood stasis syndrome. 32 rats were randomly divided into 4 groups: Group K: normal food and water (n = 8); Group L: normal feeding for 21 days and simple ligation of the left anterior descending branch ( n = 8); Y group: 21 days of diet management combined with fatigue exercise (n = 8); F group: 21 days of diet management combined with fatigue exercise + left anterior descending artery ligation (n = 8). After modeling, Photoshop 6.0 was used to analyze the RGB value of the tongue surface, and the RGB data distribution range table was used to evaluate the tongue color of each model. HE staining detects filamentous papillary epithelial layer, corneal layer, lamina propria height and lamina propria height. The number of capillaries in the lamina propria; immunohistochemical analysis of the positive expression of PGI2 and TXA2 and calculation of the T/P ratio.

　　Result: (1) The tongue quality of rats in group K is attributed to "light red tongue". The R value of the tongue of the rats in the L group was significantly lower than that of the K group belonging to the "dark red tongue". "; The R value of the tongue of the Y group is significantly lower than that of the K group, which belongs to the "white tongue"; the R, G and B values (K) and L of the F group rat tongue are significantly lower, which belongs to the "purple tongue" (2) The height of each layer of the filiform papillae of the tongue and face of the rats in the L group and the number of microvessels were not significantly different from those in the K group; the filiform papillae in the Y and K groups were significantly reduced; the filiform papillary epithelium The height of the keratinized layer was significantly reduced in the K and L groups. The number of microvessels in the K and L groups was K. More than the group. Compared with the L group, there was no difference in the height of the filamentous papilla propria in each group; (3) L, Y and F groups Compared with the K group, the PGI2 change was not significant; the P ratio of the TXA2 level group T/L group was significantly higher than that of the K group; the TXA2 level and T/P ratio of the F group were significantly higher than the K and L groups. Conclusion The RGB characteristics of tongue color in coronary heart disease syndrome with deficiency of qi and congestion are related to the decrease in the height of the filiform papillary epithelium and stratum corneum and the increase in the number of capillaries in the lamina propria. The molecular biological mechanism may be related to the imbalance of TXA2/PGI2. Etiology The comprehensive model of the model is more consistent with the characteristics of the coronary heart disease model, which combines the weakness and congestion syndrome.