动物造模,小鼠经骨髓注射肺转移和术后复发模型 z-bio 2021-10-26 318

　　Objective: To establish a model of bone marrow injection to treat lung metastasis and postoperative recurrence, to clarify the superiority and feasibility of the model established by bone marrow injection, and to provide a new research basis for the study of lung metastasis of malignant tumors.

　　Method: Divide mice into chest pad injection group, subcutaneous injection group, tail vein injection group and bone marrow injection group. We use 4T1 cells to establish different models and observe the growth, survival and metastasis efficiency of different groups of primary tumors. Inject different numbers of 4T1 cells and observe the time of lung metastasis. The postoperative recurrence model was constructed by injecting 4T1-luc cells into the bone marrow. The mice were divided into a sham cut group, a 3-day cut group, a 7-day cut group and a 10-day cut group. Observe mice by in vivo imaging.

　　Result: Compared with breast pad injection and subcutaneous injection, bone marrow injection does not affect the growth of primary tumors, but its survival time is significantly reduced. Compared with chest pad injection, subcutaneous injection and tail vein injection, bone marrow injection has the highest lung metastasis efficiency. Bone marrow injection requires only 1 x 105 4T1 cells to cause lung metastasis on the 12th day. Model mice injected with bone marrow will still cause lung metastasis and primary tumor recurrence after cutting.

　　Conclusion: We have successfully established a mouse lung metastasis model and postoperative recurrence mouse model through bone marrow injection. The mouse model using this injection method has the characteristics of short survival period and high transfer efficiency, and can be used for research.