动物造模,大鼠心肌肥大模型 z-bio 2021-11-03 300

　　OBJECTIVE: To observe and compare the effects of different genders and injection doses on myocardial hypertrophy induced by isoproterenol (ISO) in rats.

　　Method: Collect male and female SD rats and divide them into 3 groups randomly: normal group, low-dose group (1.25mg/kg) and high-dose group (5mg/kg). Ten days after subcutaneous injection of ISO, the results of echocardiography, hemodynamics, histopathology and real-time quantitative PCR were compared under the influence of various factors.

　　Results: (1) After high and low dose ISO treatment, rat heart weight parameters, ventricular septum thickness, myocardial cell area, myocardial fibrosis degree and the mRNA expression level of hypertrophic genes (ANP, BNP) increased significantly (P\u003c0.01) ), but the left ventricular function was significantly decreased (P\u003c0.01); (2) Left ventricular weight/body weight, ventricular septum thickness, left ventricular end systolic pressure change (LVESP), left ventricular maximum dilation (-dp) /dtmax) and ANP gene expression levels were dose-dependent (P\u003c0.01). Female rats only have left ventricular end systolic pressure (LVEDP), and the change in left ventricular maximum dilation (dp/dtmax) is dose-related. That is to say, the decrease of heart function in the female low-dose group was more obvious (P\u003c0.01); (3) The decrease in heart function of the female rat low-dose group was more obvious. . Male rats (P\u003c0.01); After high-dose ISO injection, the left ventricular body weight/weight and ANP gene expression of male rats were significantly higher than female rats (P\u003c0.01); (4) The survival rate was low The proportions of male rats in the dose and high-dose groups were 63.6% and 81.8%, respectively. The survival rates of the low-dose group and the high-dose group were 63.6% and 55.0%, respectively. There was no death in the normal group.

　　Conclusion: Different ISO injection doses have a significant effect on the degree of myocardial hypertrophy in male rats, but less on female rats. When modeling, male rats choose a dose of 5 mg/kg and female rats choose a dose of 1.25 mg/kg. The effect of establishing an ISO-induced rat fibroids model is relatively good.