动物模型,SLE z-bo 2020-08-03 604

　　[Modeling mechanism] The lymphocytes of activated inbred mice can be used as antigens to immunize mice of the same species, so that the host can produce its own anti-dsDNA antibodies, anti-nuclear antibodies, etc. Glomerulonephritis is similar to human lupus nephritis. Glomerulonephritis deposits antigen-antibody complexes in the kidney tissue and causes immune complexes in the kidney.

　　[Modeling method] Aseptically collect the spleen and mesenteric lymph nodes, prepare lymphocytes, and adjust the cell concentration to 2 x 1000000 cells/ml with 1640 medium, divide them into 3 groups, and prepare concanavalin. A (ConA, final concentration 10μg/ml), lipopolysaccharide (LP, final concentration 10μg/ml), IL-2 (final concentration 1000 U/ml), 37°C, 5% CO2, incubate subcutaneously for 72 hours, subcutaneously 2 ×Inject 10,000,000 cells. Count once and three times a week. Extract active nucleus and chromatin from ConA activated spleen cells, and extract resting nucleus and chromatin from unactivated spleen cells. Inject 2×10,000,000 nuclei/mouse subcutaneously once a week, chromatin 50μg, and count 3 times. After the last immunization, blood was collected from the retro-orbital venous plexus and the serum was separated. Detect IgG anti-dsDNA antibody production. Collect frozen sections of mouse kidneys and detect glomerular IgG immune complex deposition by direct glomerular detection.

　　[Model Features] ConA activated T lymphocytes, LPS activated B lymphocytes and IL-2 activated LAK cells immunized mice with high titers of IgG anti-dsDNA antibodies. The production of IgG anti-dsDNA antibodies was detected in mice immunized with active nuclei and active chromatin. Mice immunized with resting nuclei and resting chromatin did not show IgG anti-dsDNA antibodies. In mice that are positive for IgG anti-dsDNA antibodies, direct immunofluorescence can be used to detect the deposition of IgG immune complexes in the kidneys of plaque-like mice. There is no IgG immune complex in the kidneys of IgG anti-dsDNA antibody negative mice.

　　[Evaluation and application of the model] This model can be used for the pathogenesis of SLE, etiology, immune regulation of autoimmune diseases, immune complex nephritis and the pharmacokinetics of SLE treatment drugs.