动物造模,子宫内膜异位症纤维化小鼠模型 z-bio 2021-11-10 300

　　Objective To explore an efficient method for establishing and evaluating a mouse model of endometriosis fibrosis.

　　Methods A modified intraperitoneal injection method was used to construct a BALB/c mouse endometriosis fibrosis model; the mouse peritoneal lavage fluid was collected, and the ELISA kit was used to detect the level of TGF-β1 in the lavage fluid; the degree of abdominal adhesion of the mice was scored ; HE staining to verify the success of the endometriosis model; Masson staining to detect the deposition of collagen fibers in the ectopic foci and to evaluate the degree of collagen deposition; immunohistochemistry to detect the fibrosis-related proteins E-cadherin, Collagen I, and α-SMA 、The expression of smooth muscle myosin heavy chain II (SMMHC-II) to evaluate the degree of ectopic focus fibrosis.

　　Results The success rate of the modified intraperitoneal injection method to construct a mouse model of endometriosis fibrosis in mice can reach 100%. The level of TGF-β1 in mouse peritoneal lavage fluid increased significantly with the prolonging of the modeling time. Masson staining and fibrosis-related protein immunohistochemical staining showed that the abdominal cavity of mice with endometriosis had a fibrotic phenotype.

　　Conclusion The improved intraperitoneal injection method has a high success rate in constructing a mouse endometriosis fibrosis model. The level of TGF-β1 in peritoneal lavage fluid, Masson staining and fibrosis-related proteins E-cadherin, Collagen I, α-SMA, SMMHC -II Immunohistochemical staining can evaluate the degree of pelvic fibrosis in mice with endometriosis.