动物造模,局灶性脑缺血模型 z-bio 2021-11-26 601

　　Objective: To coagulate the middle cerebral artery in mice, observe the feasibility of electrocoagulation to cause middle cerebral artery occlusion, and provide the possibility to establish a mouse cerebral ischemia model.

　　Method: Use electrocoagulation to directly block the middle cerebral artery (middle cerebral artery, MCA) to create an adult male Balb/c mouse cerebral ischemia model (model group, n = 20). At the same time, create the same batch of Balb/c. The mouse craniotomy was the same, but mice without electrocoagulation and middle cerebral artery occlusion were regarded as the sham operation group (sham operation group, n = 20). In the model group and the sham operation group, the neurological function score (mNSS) was used to evaluate the nerve damage at 24 and 72 hours after surgery.

　　Result: Twenty-four hours after the operation, a slice of the brain tissue of the injured part of Balb/c mice in the model group was taken, and hematoxylin-eosin was stained. Microscopic examination showed ischemic brain tissue interstitial edema. , Cavities are formed, loose light color with local brain tissue structure, the number of nerve cells is significantly reduced, the cell body of nerve cells shrinks, and the outline of some cells is not clear. There are varying degrees of cytopathic and necrosis, abscesses and nucleolar loss. After 24 hours, the neurological deficit score of the model group was significantly lower than that of the sham operation group (P\u003c0.05), and this difference can be maintained until 72 hours after surgery.

　　Conclusion: Electrocoagulation can successfully create a mouse model of local cerebral ischemia in Balb/c mice.