抑郁症模型,动物实验 z-bio 2021-02-23 271

　　Objective: To establish a long-term stress-stimulated animal depression model.

　　Method: Choose 17C57BL/6J 4-week-old mice weighing 4 weeks (11.05±1.65) g, and randomly divide them into a control group and a long-term psychological stress stimulation (LTMS) group. The LTMS group was kept in a cage and fed the cats daily for 21 days, including 3 hours of electric shock and 12 hours of sleep deprivation. The experimental group determined the success of the modeling by measuring the changes in food intake, the increase in cross-maze experiments, field experiments, and changes in forced swimming behavior. Compared with the results, the food intake of the LTMS group was significantly reduced (P \u003cu003c0.01), and the time of forced swimming immobility was significantly prolonged (P \u003cu003c0.01). In the field test, the number of crossing grids and the number of posts in the LTMS group were significantly less than those in the control group, and the difference was very significant (P\u003c0.01). In the elevated plus maze experiment, the residence time of the closed arm in the LTMS group was significantly longer, and the difference was very significant (P\u003c0.01), while the rate of entering the open arm was reduced, and the difference was large.

　　Conclusion: The long-term psychological stress model shows desperate behavior, decreased mobility, loss of interest, anxiety and similar depressive symptoms, and can be used as a depression model.