动物造模,颅脑损伤后认知障碍大鼠模型 z-bio 2021-11-26 505

　　Objective: To discuss the preparation and evaluation of animal models of cognitive dysfunction after traumatic brain injury.

　　Method: 60 healthy male rats (Sprague Dawley, SD) and 48 water mazes were screened through experiments, and they were randomly divided into blank control, model control and model group. This model uses the electronic CorticalContusionImpactor (eCCI) combined with maze water screening to identify rats with cognitive impairment after brain injury (cranial brain injury, TBI). Observe the vital signs, nerve function and motor function of the rat, perform Morris water maze experiment, and measure the changes in serum MMP-2, MMP-9, SOD and MDA content.

　　Result: Compare with the blank group. The vital signs of the model group were stable and the behavior indicators were different (P\u003c0.05). There are significant differences in the latency results of the water maze experiment. Space exploration experiment (P\u003c0.01). The levels of serum MMP-2, MMP-9 and MDA increased significantly (P\u003c0.01), and SOD decreased significantly (P\u003c0.01).

　　Conclusion: This model preparation strategy can better simulate the cognitive impairment after head injury, while providing stability, repeatability, objective evaluation and ease of operation. It can be used as a reference for similar research.