大鼠肺动脉高压模型 z-bio 2021-02-23 327

　　Objective: To investigate the expression level and role of RhoA/ROCK signaling pathway in a rat model of left heart disease-related pulmonary hypertension.

　　Method: Twenty male SD rats weighing 90-100 grams, aged 3-4 weeks, were randomly divided into a control group (group C: n = 10) and a pulmonary hypertension group (group H: n = 10). In group H, a rat model of pulmonary hypertension associated with left heart disease was established by fixing and contracting the ascending aorta. Rats in group C were treated with sham operation (fixed titanium clamps instead of paravascular mediastinum tissue; ascending aorta; all other operations are the same as those in group H), and hemodynamics were performed on each group of rats 60 days after modeling Test (right ventricular systolic pressure, pulmonary artery pressure), and examine the heart and lungs in vivo at the expense of rats. Washing with PBS can make the lungs white, and fix the left lung tissue with 4% paraformaldehyde to observe the pathological changes of the lung tissue. Frozen right lung tissue is used for biomolecular detection (Rho kinase mRNA, RhoA mRNA, ET-A receptor mRNA).

　　Results: Compared with group C, pulmonary artery pressure and right ventricular systolic pressure in group H were significantly increased (P\u003c0.01), the pulmonary arteriole wall was significantly thickened, the pulmonary arterioles were narrowed, and the wall hypertrophy index was significantly increased , Even with large obstacles (P\u003c0.01); compared with group C, the expression level of Rho kinase mRNA in the lung tissue of group H was significantly increased, and the expression level of RhoA mRNA and ET-A receptor mRNA was also significantly increased. high. Significantly (P\u003c0.01).

　　Conclusion: A rat model of left heart disease-related pulmonary hypertension was successfully established through the fixation and contraction of the ascending aorta. Compared with group C, small pulmonary vessel walls in group H were significantly thickened, and the expression of Rho kinase mRNA, RhoA mRNA and RhoA mRNA and ET-A receptor mRNA in lung tissue were significantly increased, which may be related to signal transduction pathways. Pulmonary hypertension associated with left heart disease is formed.