动物造模,兔脑死亡模型 z-bio 2021-02-23 383

　　Objective: To explore a method to establish a rabbit brain death model that improves and maintains brain death in intracranial pressure.

　　Methods: 15 New Zealand white rabbits were randomly divided into 2 groups: sham operation group (group B, n = 6), only intracranial intubation, anesthesia; brain death group (group A, n = 6) 9), using modified Intracranial compression established a brain death model. Record mean arterial pressure (MAP) and heart rate (HR) changes. Ventilators and vasoactive drugs are used to maintain vital signs at specific and controllable levels.

　　Result: 8 of the 9 rabbits in group A were successfully modeled. With the increase of intracranial pressure during intracranial pressure, MAP and HR gradually increase like waves. The peak values of MAP under anesthesia and brain death were (80.63±8.45) mmHg and (111.63±7.71) mmHg, respectively, which were statistically significant (P\u003c0.05). Under anesthesia, HR was (153.25±14.35) times/min, (262.38±16.60) times/min and (218.50±10.27) times/min at the time of brain death and 2 hours after brain death. Statistical difference (P\u003c0.05).

　　Conclusion: Compared with traditional surgery, the improved method can establish a stable and reliable rabbit brain death model. Timely and effective breathing and circulatory support can help maintain brain death for a long time.