动物造模,小鼠骨肉瘤模型 z-bio 2021-02-23 377

　　Objective: To explore the key factors affecting the success rate of animal models of osteosarcoma.

　　Method: 24 C3h mice aged 4-8 weeks were randomly divided into two groups, including 6 types of C3h/HeJ and 18 types of C3h/HeN. In group A, after depilation with ordinary depilatory cream, 0.2 mL of LM8 cell suspension was injected into the lateral armpit at a concentration of 5 x 107 digits/mL. In group B, the concentration after shaving with a subcutaneous injection of concentrated animal razor in the lateral armpit was 5×107. Locally massage 0.2 mL of LM8 cell suspension to the injection site in the order of 1 mL every day, and record the growth of the tumor.

　　Result: The proportion of tumor group is 33% (4/12), and the proportion of tumor group B is 100%. Conclusion: Various injection techniques are very important for LM8 carcinogenesis and subsequent tumorigenesis.