动物造模,腰椎板开窗术后微小骨粒回植模型 z-bio 2021-02-23 391

　　OBJECTIVE: To establish a rabbit model of bone replantation after opening the window of the lumbar vertebral lamina in rabbits to provide experimental evidence for clinical trials.

　　Method: Divide 18 New Zealand male rabbits into two groups. In the control group, after general anesthesia, the L5 spinous process was centered, the left L5 lamina was completely exposed, the lamina and ligamentum flavum were slowly bitten with a microgun, and a window was opened on the approximate bone. The window was exposed. 0.8x0.3 cm size. Immediately after stitching. After opening the window on the left side of L5, the experimental group cut off the bone fragments bitten by the window, put the bone fragments into a medical collagen sponge to form a bow-shaped bridge, and placed it on the epidural window and sutured it. After the operation, the histological changes of CT and the position of the lamina opening were observed.

　　Result: Computed tomography showed that 8 weeks after the operation, small bone particles formed a thin bone plate at the opening of the experimental group. After 12 weeks, the bone plate formed became thicker, and the trabecula was continuous in the shape of a spinal canal. There is no significant change in volume. The spinal cord is compressed. The control group had a small amount of bone hyperplasia at 8 and 12 weeks after the window was opened, and the surrounding adhesion tissue infiltrated the spinal canal, but the spinal canal was not reconstructed.

　　Conclusion: By implanting autologous small bone particles into the opening of the rabbit lamina to reconstruct the bone, it can prevent the scar from invading the spinal canal and reduce the adhesion of the spinal canal.