动物造模,系统性感染模型 z-bio 2021-10-28 407

　　Objective: To study the oral Candida infection in ICR mice, establish a systemic infection model, and observe the proliferation and distribution of Candida in mouse tissues after mucosal infection.

　　Method: 46 ICR male mice were randomly divided into model group A (n = 20), model group B (n = 20) and control group (n = 6). The model group was orally administered Candida albicans (7×106 cfu/mL) by the cotton swab method, and the control group was also inoculated with the same amount of normal saline. Model A mice were used for clinical, survival and autopsy observation experiments. On the 3rd, 5th and 7th day after inoculation, model B group randomly dissected 5 mice for tissue load and pathological examination (each time point in the control group) 2. Dissected the animals, and detected the tissues in the mouse tissues by the survival plate counting method. Contain the amount of bacteria, and observe the histopathological changes of the tongue, stomach, liver and kidney of the mice through an optical microscope.

　　Result: Three days after inoculation, mice in the model group showed obvious white spots on the tongue surface, which increased with the passage of time and died. On the 5th day, the mortality rate of the mice exceeded 50%, and on the 7th day, the mortality rate reached 100%. Candida albicans was isolated from 87.5%), stomach (87.5%), internal organs, liver (54.5%), kidney (50.5%), lung (20%) and heart (4%). Observed under a microscope in the tongue, esophagus and stomach, liver and kidney showed sclera growth, while the control group did not show Candida albicans growth. This indicates that the model group of mice caused by Candida albicans mucosal infection caused diffuse systemic infection in the mice.

　　Conclusion: C. Under certain conditions, Candida albicans can break through the mucosal immune barrier and cause opportunistic systemic infections in mice, thereby exacerbating infection and death