动物造模,胃癌原位移植模型 z-bio 2021-10-13 317

　　Objective: To study the application effect of heptamethine cyanine near infrared fluorescence (NIRF) dye in in vivo imaging of orthotopic transplantation model of gastric cancer.

　　Method: The fluorescein-labeled human gastric cancer cell line HepG2 was transplanted into nude mice in situ to establish a tumor model and induce the establishment of a gastric ulcer model. Bioluminescence imaging and NIRF imaging are used in the above models to study the effects of hypoxia and anion transport peptide (OATP) on the absorption of NIRF dyes by gastric cancer tissues (mainly gastric cancer tissues). We will clarify the specificity of NIRF dyes for gastric cancer tissues. Target and identify tumor cells.

　　Result: NIRF and bioluminescence signals have a good correlation in in vivo imaging of orthotopic transplantation models of gastric cancer. A strong NIRF fluorescence signal was obtained in gastric cancer tissue, but no fluorescence signal was detected in gastric ulcer. Hypoxia can increase the absorption of NIRF pigment by gastric cancer cells, and the anion transit peptide specific inhibitor sodium sulfobromide (BSP) can significantly reduce the absorption of NIRF pigment by tumor cells.

　　Conclusion: Qijiachuanjing NIR fluorescent dyes can be targeted and identified in orthotopic transplantation models of gastric cancer.