动物造模 z-bio 2021-11-04 279

　　OBJECTIVE: To establish an SD rat vulvar chronic lichen planus (LSC) animal model, to study the expression of protease activated receptor 2 (PAR2) in vulvar lesions of model rats, and to provide a basis for an ideal LSC animal model.

　　Method: 70 female SD rats were randomly divided into group A (blank control group, n = 10), group B (acetone control group, n = 10), and group C (mechanical stimulation control group, n = 10). enter. Group D (experimental group, n = 40). Group A received no treatment; Group B applied acetone solution on the rat genital skin 3 times a week for 10 weeks; Group C performed simple mechanical stimulation on the rat skin 3 times a week for 10 weeks; Group D used 0.5 % Dimethylbenzanthracene (7,12-dimethylbenzanthracene, DMBA) acetone solution was combined with mechanical stimulation three times a week to induce rat genital skin for up to 10 weeks. At 6, 8, 10, 12 and 14 weeks, the skin changes of the rat's vulva were observed with naked eyes and under a microscope. Immunohistochemistry and Western blotting were used to detect the expression of PAR2 protein in groups A and D, and qRT-PCR to detect the expression of PAR2 mRNA.

　　Results: 57.5% (23/40) of SD rats in group D had LSC changes at the 8th week, and 95% (38/40) of the vulva showed LSC at the 10th week. By 12 weeks, 20% (8/40) of the rats had papilloma in the vulva. Acetone and simple mechanical stimulation will not affect LSC. The results of immunohistochemistry and Western blotting showed that the PAR2 protein expression in group D was significantly higher than that in group A (P\u003c0.05). The qRT-PCR results showed that the mRNA expression of PAR2 in group D was significantly higher than that in group D. Group A (P\u003c0.05).

　　Conclusion: Mechanical stimulation combined with 0.5% DMBA acetone solution can induce LSC in female SD rats. PAR2 may be closely related to the occurrence and development of diseases.