动物造模,肺腺癌脑转移动物模型 z-bio 2021-11-06 283

　　Objective: To establish a biological model of luc + -PC-9 human lung adenocarcinoma cells stably expressing luciferase, bioluminescence imaging and 18F-FDG (18F-fluorodeoxyglucose, 18F-fluoro) deoxyglucose to establish brain metastases from lung cancer Animal model.

　　Method: Inject luc + -PC-9 cell suspension from the left ventricle into BALB/c nude mice to establish a lung cancer brain metastasis model, and perform bioluminescence imaging and 18F-FDGSPECT at 4 and 5 weeks. carried out. In order to observe the growth tumor status of nude mice, the pathological results of H&E staining were used as the gold standard to compare the effects of these two methods in lung cancer metastasis models.

　　Result: A brain metastasis model of lung cancer was established by injecting luc + -PC-9 cells into the left ventricle. The success rate of brain metastasis is 85%. The number of tumor cells is positively correlated with the luminescence intensity, and has a good linear relationship (R2 = 0.96). Bioluminescence imaging can observe the fluorescent signals of the brain, spine and femur, and pathological results confirm that this is a metastasis. 18F-FDGSPECT/CT did not find obvious metabolic lesions in the brain tissue, but showed metabolic lesions in the femur or spine, and showed pathologically confirmed bone marrow metastasis.

　　Conclusion: Left ventricular injection is a reliable method to establish a brain metastasis model of human lung adenocarcinoma. The bioluminescence imaging system has high sensitivity and specificity for detecting brain and bone metastases, and can observe the growth of metastases in real time, dynamically and non-invasively. 18F-FDGSPECT/CT has no advantage in detecting brain metastases.