动物造模,哮喘小鼠模型 z-bio 2021-11-06 285

　　OBJECTIVE: To observe the role of interleukin 22 (IL-22) in asthma models, and to study the effect of budesonide on airway inflammation and IL-22 regulation in asthmatic mice.

　　Method: 24 BALB/c mice were randomly divided into three groups: control group, asthma model group and budesonide group, and ovalbumin (OVA) was used to sensitize and stimulate the mice to stimulate the asthma model. create. Use HE and AB-PAS to stain mouse lung tissue to record airway inflammation, use ELISA to detect IL-22 levels in mouse alveolar lavage fluid (BALF), and use real-time fluorescent quantitative PCR, and use mouse lungs Tissue-22 mRNA expression level.

　　Result: Compared with the control group, the lung tissue inflammation score of asthmatic mice increased, the level of BALF IL-22 and the level of IL-22 mRNA in the lung tissue increased. This difference is statistically significant. After budesonide treatment, the airway inflammation score and IL-22 mRNA expression in the lung tissue of the mice were lower than those in the asthma group, and the difference was statistically significant.

　　Conclusion: The therapeutic effect of budesonide on airway inflammation in asthma is related to the inhibition of IL-22 expression and lung secretion.