动物造模,小鼠慢性骨盆疼痛综合征模型 z-bio 2021-02-25 663

　　OBJECTIVE: To establish a C57BL/6 mouse model of chronic pelvic pain syndrome prostatitis and its mechanical pain threshold and the expression of autophagy-related microtubule light chain protein LC3 and substrate protein. The model time law provides an experimental basis for animal studies on CP/CPPS pain and autophagy levels.

　　Method: 36 male C57BL/6 mice were randomly divided into blank group, control group and model group. The mice in the model group were injected subcutaneously with a suspension of rat prostaglandin extract and complete Freund's adjuvant at multiple points. CP/CPPS mouse model. HE staining was used to observe the pathological changes of the prostate, VonFrey fiber was used to determine the mechanical pain threshold in the pelvic area, and the expression levels of LC3 and p62 were detected by immunohistochemical staining. The average optical density was ImageProPlus 6.0. software.

　　Results: HE staining showed that the model group mice developed chronic prostatic inflammation, showing varying degrees of epithelial hyperplasia and lymphocyte infiltration, and 6 months after the experiment, prostate intraepithelial neoplasia (PIN) appeared in the prostate, and showed Basement membrane. Obviously disappeared and nuclear atypia, blank and control group showed normal tissue morphology. Compared with the blank group and the control group, the mechanical pain threshold of the model group gradually decreased with the extension of modeling time [initial pain threshold was (0.35±0.154)g, 22 weeks was (0.008±0.000)g. Yes], the difference is significant (P\u003c0.05). The expression levels of LC3 and p62 gradually increased [the average optical density values of LC3 and p62 were (2.767±0.464)% and (2.872±1.642)%, respectively; the sixth month: (13.501±), respectively. 1.900)%, (9.070±0.490)%], the difference is significant (P\u003c0.05).

　　Conclusion: The CP/CPPS model was successfully established, and the PIN was displayed 6 months after the model was established. The mechanical pain threshold of mice in the model group gradually decreased with the increase of modeling time, the expression of LC3 and p62 gradually increased, the CP/CPPS inflammatory microenvironment promoted the onset and aggravation of pain, and autophagy in the mouse prostate Development is closely related to the development of PIN.