动物造模,小鼠肝癌模型 z-bio 2021-11-29 735

　　Objective: To establish a mouse liver cancer model induced by diethylnitrosamine (DEN), and use two formula foods: SPF grade mice and rats complete compound diet and AIN93-G combined synthetic diet to establish an effect induction Mouse liver cancer model. DEN-Explore different eating habits.

　　Method: A mouse liver cancer model was established by injecting 25 mg/kg body weight of DEN solution into the abdominal cavity of 14-day-old male C57BL/6 mice. After weaning, the mice were divided into two groups. One group was fed regular SPF-grade regular-price formula food for rats and mice, and the other group was fed AIN-93G formula artificial food. The model mice were killed at 9 months of age, and liver tissues were harvested and weighed. The growth of liver cancer was observed and recorded.

　　Result: All mice in the complete diet group had tumors. The body weight and liver weight of mice in the AIN-93G group were significantly lower than those in the complete diet group. The incidence, number and size of liver cancer and the proportion of tumor nodules were also significantly lower than that of the complete diet group. Mice in the price feed group.

　　Conclusion: The development of liver cancer induced by mice fed with AIN-93G diet was suppressed and their body weight was significantly suppressed, while mice fed with DEN-induced diet completely suppressed their body weight. Liver cancer was successfully established. It reduces and plays an important role in model establishment, indicating that dietary factors are related to animal diseases.