动物造模,小鼠间断性禁食模型 z-bio 2021-02-25 333

　　Objective: To study the stable and reliable alternatives to traditional calorie restriction in mouse models and their effects on blood glucose homeostasis.

　　Method: 40 SPF 16-week-old C57BL/6J mice (half male and half male) were randomly divided into 4 groups according to gender and feeding method. The free-eating group can eat the basic diet freely, while the intermittent fasting group can alternate between normal and fasting for 24 hours, and drink freely on fasting days. The body weight and blood glucose levels of each group of mice were monitored, and the transabdominal glucose tolerance test and the transabdominal insulin tolerance test were performed before and 12 weeks after the modeling.

　　Result: After 12 weeks of intermittent fasting, the body weight and fasting blood glucose of the mice did not change significantly. After intraperitoneal injection of glucose, the blood sugar level rises and decreases, and after intraperitoneal injection of insulin, the proportion of blood sugar levels decreases. Compared with the control group, the area under the resistance experiment curve was significantly reduced (P\u003c0.05).

　　Conclusion: After intermittent fasting, mice become more sensitive to insulin and impaired glucose tolerance. This modeling method is easy to operate and is an effective alternative to the traditional calorie restriction model.