动物造模,肠系膜上静脉血栓形成大鼠模型 z-bio 2021-02-25 266

　　Objective: To establish a rat model of superior mesenteric venous thrombosis by ligation, to simulate the pathological process of the disease, and to provide basic preparations for the study of its pathogenesis and treatment methods.

　　Methods: Ninety-six male SD rats were randomly divided into three groups, group A (sham operation group), group B (strangulated group) and group C (simple group), with 32 rats in each group. Rats in group A only opened the abdominal cavity without blocking blood supply. Rats were sacrificed in batches at 8, 24, 48, and 72 h after surgery; rats in group B and C were ligated to establish a strangulated superior mesenteric vein thrombosis. Rats were sacrificed in batches at 8, 24, 48, and 72 h after modeling and simple model. HE staining was used to observe the morphological changes of the rat’s intestinal tissues and score the damage; ELISA was used to detect the levels of rat serum midgut fatty acid binding protein (IFABP) and α-glutathione S transferase (α-GST).

　　Results: The results of HE staining and pathological scoring showed that compared with the rats in group A, rats in group B and group C had different degrees of blood stasis and damage in the intestinal tissues, group B gradually aggravated, and group C gradually reduced, and The degree of blood stasis and injury is positively correlated with the extent of ligation. The results of the serum ELISA method showed that compared with the rats in group A, the levels of IFABP and α-GST in the serum of rats in group B and C increased to different degrees (P<0.05), and the degree of increase was positive with the range of ligation Related.

　　Conclusion: This study used ligation to successfully establish a rat model of superior mesenteric vein thrombosis, which is simple and easy to perform and has a high surgical success rate. It can be used in related studies.