动物造模,子宫内膜异位症盆腔粘连大鼠模型 z-bio 2021-02-26 484

　　Objective: To explore the method and evaluation index of establishing a rat model of endometriosis with pelvic adhesions.

　　Method: Autologous transplantation is used to transplant the endometrium into the rat mesentery. Eight rats were randomly selected on the 1, 3, 5, 7, 14, 21, and 28 days after the operation to observe the growth of the lesion. This status has been adopted. The degree of pelvic adhesion is scored by the Society for Reproductive Society (AFS) adhesion score (AFS) and the harbor adhesion score. At the same time, each group of lesions and surrounding endometrial tissues are used. For HE staining, the adherent tissue around the lesion was collected at 5, 7, 14, 21, and 28 days after surgery to dynamically determine the content of tissue plasminogen activator (tPA).

　　Result: Both adhesion scoring methods showed that typical pelvic adhesions were formed 5 days after modeling. Compared with the blank control group and the sham operation, the tPA content of the pelvic adhesion peritoneum tissue of each model group was significantly reduced after 5 days. Modeling (P\u003c0.01), 28 days after modeling, tPA content showed an upward trend, but it was still significantly lower than the blank control group and the sham operation group (P\u003c0.01).

　　Conclusion: The EMs pelvic adhesion model was established by autotransplanting the endometrium into the mesentery. The mature model is 5 days after modeling. There is a correlation between tPA and EMs pelvic adhesions.