动物造模,大鼠肺炎模型 z-bio 2022-01-10 287

　　Objective: To explore the establishment of a rat model of Pseudomonas aeruginosa pneumonia, and to provide experimental evidence for follow-up research.

　　Method: 48 Sprague-Dawley (SD) rats were randomly divided into 4 groups: control group, B tracheal injection group, C tracheal intubation infection group, and D rhinorrhea infection group. After adapting to feeding for 3 days, various modeling interventions were performed to dynamically change the weight, temperature, white blood cell count, and pathological changes of lung tissue in each subgroup within 5, 10, and 15 days.

　　Results: The behavioral ability, body temperature, body temperature, white blood cell, inflammation and pathological ability of rats in each model group were significantly different from those of the control group; ②Each model group was infected with Pseudomonas aeruginosa. Well, the control group is negative.

　　Conclusion: Infection of Pseudomonas aeruginosa through the nasal route can provide a high-quality pneumonia model for rats. The transmission route avoids the interference of the inflammatory response caused by the surgical wound, and is simple, easy to implement and convenient.