6-羟基多巴模型大鼠,动物造模 z-bio 2021-02-26 354

　　Objective: To establish a rat model of Parkinson's disease using 6-hydroxydopamine (6-OHDA) and to detect salsolinol nitrogen methyltransferase (SNMT) in peripheral blood lymphocytes of rats.

　　Method: Use 6-OHDA unilateral two-point injection to damage rat striatum, establish a PD model, and evaluate the behavior of the model. The crude SNMT enzyme solution was extracted from the peripheral blood lymphocytes of model rats to establish the N-methyl group of the enzyme reaction product. -Salsolinol (NMSal) is a high performance liquid chromatography tandem triple quadrupole mass spectrometry method, which characterizes the activity of SNMT by the amount of NMSal produced.

　　Result: A total of 7 rats, namely 18 6-OHDA-injected rats, continued to rotate on the uninjured side after apomorphine induction (7r/min), and the modeling success rate was 38.9%. Established a high selectivity, high sensitivity and high reproducibility characterization method for SNMT activity based on multi-reaction monitoring technology. The detection limit and quantification limit of this method for NMSal reached 49 pmol/L and 98 pmol/L, respectively, and the accuracy during day and day was less than 6.0%. The test results showed that the SNMT activity of peripheral blood lymphocytes in the PD group was significantly different from that of the sham operation group and the normal group (P0.05, n = 5).

　　Conclusion: The SNMT activity of peripheral blood lymphocytes may reflect the development of PD and is expected to become one of the diagnostic indicators.