动物造模,氟中毒大鼠模型 z-bio 2021-02-26 285

　　Purpose: How to establish an animal model of male and female fluorosis rats and study the gender differences in fluorosis?

　　Method: Select 48 SD rats weighing about 150 g and divide them into a control group and a fluoride exposure group. The model is free. The fluorine content in the feed that eats fluorine-containing feed is 100 mg/kg. Dynamic observation of the condition, weight and daily changes of fluorosis every three days. When all women develop fluorosis, blood is drawn from the femoral artery. Rats and 110-day male rats. Is a batch of rats using fluoride ion selective electrode method to detect fluoride in urine and fluoride in bone? Results Neither female nor male rats in the control group showed dental fluorosis. The whole experiment? Male and female rats in the fluoride exposure group first developed obvious fluorosis about 60 and 80 days after fluoride exposure. Fluorosis first appeared on the teeth of male rats after about 20 days. About 30 days after being exposed to fluoride, the male and female rats entered the rapid growth stage, and the weight difference between male and female rats was significant (P\u003c0.01)? Are male and female rats exposed to fluoride for 110 days heavier than the control group after long-term exposure to fluoride? With the prolongation of fluorine exposure time, the urine fluoride and bone fluoride of male and female rats gradually increased, which was significant in the fluoride exposure group (P\u003c0.01), and the urine fluoride and bone fluoride were exposed to fluoride Chemistry, is this positively related to time? Male rats have higher urine fluoride and bone fluoride content than females, and the difference is statistically significant (P\u003c0.01). The effect of fluoride on urinary fluorosis and bone fluorosis. Gender influence?

　　Conclusion: What are the body weights of male and female rat models suffering from fluorosis? When does fluorosis appear? Are there gender differences in fluoride levels between fluorosis and bones?