动物造模,大鼠宫内发育迟缓模型 z-bio 2021-10-26 271

　　Purpose: Is intrauterine growth restriction (IUGR) manifested in the form of low birth weight? The IUGR model is established through three commonly used experimental methods, and the IUGR model in scientific research is established through the comparison and analysis of experimental data.

　　Method: On the 0th day of pregnancy, 20 pregnant mice were randomly divided into 4 groups, 5 mice in each group, 3 of which received alcohol intervention (A)? Uterine artery ligation (U)? Using protein diet (LP) and other methods to establish a low IUGR rat model, the fourth group is the normal control group (CON). The fetus was taken from a caesarean section on the 20th day of pregnancy. We measured the body weight and brain mass of fetal mice. It measures the quality of the placenta and the quality of the kidneys, measures the length of the body and the length of the tail, and calculates the incidence of IUGR, the survival rate and specific gravity of the brain, and the specific gravity of the fetus. placenta. Wait; does each group continue intrauterine growth retardation randomly within 3 to 6 weeks? Do you want to weigh within 12 weeks? Kidney fat mass and some organ mass?

　　Results: The average body weight of 3 people received alcohol intervention, the uterine artery ligation and low-protein diet fetal mice group was significantly lower than the normal control group (P0.05); 12 weeks after feeding, the average body weight of the rats in the intervention group and the low-protein diet group Drinking alcohol is significantly higher than the normal control group (P\u003c0.05)?

　　Conclusion: Among all three modeling methods that can successfully establish an IUGR model, the low-protein diet group is a high-incidence, low-birth-weight IUGR animal model suitable for model research. From the perspective of catching up with growth, people who have cesarean section and postpartum lactation should use alcohol intervention and a low-protein diet. The average body weight is lower than the normal control group, but the growth rate after lactation is much higher than that of the normal control group. Does this indicate that the two experimental groups have caught up with the growth?