动物造模,卵巢早衰动物模型 z-bio 2021-02-26 337

　　Objective: To compare different injection methods to establish an animal model of primary ovarian insufficiency induced by cyclophosphamide, find the best modeling method, and provide a more suitable experimental model for further study of primary ovarian insufficiency.

　　Method: 21 rabbits were randomly divided into 4 groups, 3 in the normal control group, no treatment (group A), 6 in the model group, injected with 50 mg/kg cyclophosphamide solution every day (group B). The second group, 50 mg/kg. Continuous injection for 2 days (group C); 6 rats in the 3 groups of models were injected with 50 mg/kg on the first day, and then at 8 mg/kg for 14 consecutive days (group D)? The rabbits were weighed, and the ovaries and body weight of each group were described within 14 days after injection. Change, analyze the ovarian index, observe the shape of follicles by HE staining, count the number of follicles at various levels under an optical microscope, observe the apoptosis and analyze the cells by the TUNEL method; serum estradiol (E2)) analyze its changes by ELISA?

　　Result: Weight: B? There was no significant change in body weight in group C (P\→0.05); growth restriction of rabbits in group D (P0.017)? In the three groups A, B and C, there was no significant difference in the abnormal rate of secondary antral follicles (P\→0.05). Apoptosis rate: Apoptosis mainly occurs in granuloma cells of follicles. The apoptosis rate of C cells in group B was significantly higher than that of group A (P\u003c0.05), the apoptosis rate of group C was higher than that of group B (P\u003c0.05) 0.05, serum E2 value: E2 secretion in group B After the 7th day and the 7th day, it gradually decreased, and the E2 secretion on the 7th day was significantly higher than that on the 35th day (P\u003c0.05); the E2 level of group C continued to decline, and the 0th day was significantly higher than the 3rd day. 35 (P\u003c 0.05)?

　　Conclusion: Is 50 mg/kg continuous injection for 2 days the best way to establish a rabbit model of early ovarian failure induced by cyclophosphamide?