慢性阻塞性肺疾病模型 z-bio 2021-02-26 311

　　Objective: To observe the effect of sodium houttuyfonate on the expression of PI3K, AKT1 and mTOR mRNA in the lung tissue of rats with chronic obstructive pulmonary disease, and to explore its mechanism of action.

　　Method: Twenty-four Wistar male rats weighing (220±20) g were randomly divided into normal group, model group, dexamethasone group and longevity sodium group (6 animals in each group). The combination of smoking and lipopolysaccharide tracheal infusion established a rat model of chronic obstructive pulmonary disease (chronic obstructive pulmonary disease, COPD). The expression of PI3K, AKT1 and mTOR mRNA was detected by real-time fluorescent quantitative polymerase chain reaction. Rat lung tissue was observed. Lesions.

　　Results: Compared with the normal group, the expression of PI3K and AKT1 mRNA in the lung tissue of the model group was significantly increased (P\u003c0.01, P\u003c0.05), and the expression of mTOR mRNA was significantly increased. Decrease (P\u003c 0.01); Compared with the model group (P\u003c0.01), the expression of PI3K and AKT1 mRNA was significantly increased; the expression of PI3K and dexamethasone in the lung tissue was significantly decreased (P\u003c0). .01, P\u003c0.05), compared with the dexamethasone group, the expression of mTOR mRNA was significantly increased (P\u003c0.01); the expression of mTOR mRNA in the lung tissue of the sodium group was significantly increased (P\u003c0.01 ) (P\u003c0.01). u003c0.05). Pathological observations showed that compared with the normal group, the model group had local lung consolidation, a large number of neutrophils infiltrated the alveolar space, collagen staining showed a large number of lung interstitial fibrous tissues, showing hyperplasia; lung tissue pathological changes. The houttuyfonate sodium group and dexamethasone group were much lighter than the model group. The lung tissues of the sodium sodium sodium group and dexamethasone group showed mild interstitial pneumonia, and the growth of fibrous tissue was lighter.

　　Conclusion: Sodium houttuynate can reduce lung tissue damage in rats with chronic obstructive pulmonary disease. The mechanism may down-regulate the expression of PI3K and AKT1 mRNA, and up-regulate the expression of mTOR mRNA, which may be related to the ability.