动物造模,全子宫切除模型 z-bio 2021-12-01 329

　　Objective: How to establish a BALB/c mouse total hysterectomy experimental animal model, and investigate and analyze the characteristics and formation mechanism of the model.

　　Method: Divided into blank control group, sham operation group and model group. The blank control group did not receive any treatment. Diet; the model group underwent a total hysterectomy through the abdomen; did the sham operation group just cut the skin to expose the uterus? The mice were reared regularly after the operation, and the weight changes of the mice were dynamically observed. At the end of 4 weeks, the peripheral blood estradiol content and ovarian tissue were measured.

　　Result: All experimental mice in each group had good general symptoms, the incision had no obvious inflammation, and they were completely cured; there was no significant difference in the weight of the experimental mice in each group at 2 weeks after the operation. In the 3rd and 4th weeks, the body weight of the mice in the model group exceeded that of the control group. Peripheral blood estradiol content in sham operation group and sham operation group (P\u003c0.05); significantly lower than control group and sham operation group (P\u003c0.01); ovarian histology and blank control group sham operation group No abnormalities. Do these models show abnormal histological changes, such as irregular follicular morphology, loose granular cells, and apoptosis?

　　Conclusion: The experimental animal model can be simulated well clinically. Are postoperative ovarian dysfunction and other clinical manifestations an ideal animal model for studying complications after hysterectomy and screening drugs?