动物造模,大鼠脓毒症模型 z-bio 2021-12-01 244

　　Objective: How to study the changes in blood coagulation function of septic rats caused by pus puncture (CLP)?

　　Method: Using CLP to induce sepsis in SD rats, the coagulation function index was detected by HE staining 8-16 hours and 48 hours after the operation, the correlation between lung, kidney, liver and spleen tissues, have you observed pathological changes?

　　Result: Is the 12-day survival rate of CLP-induced septicemia rats 30%, indicating an acute attack and high mortality? In the acute phase of development, the APTT time of CLP rats was extended by 8 hours (P\u003c0.05), the PT time was extended by 16 hours (P\u003c0.05), and the XII activity and the external coagulation pathway of the endogenous Transient inhibition of sexual VII active coagulation pathway? Will TT be extended within 48 hours (P\u003c0.01)? The FIB content gradually increases from 16 hours (P\u003c0.001)? The number of other important indicators related to blood coagulation and anticoagulant function, PLT, gradually decreased from 8 hours (P\u003c 0.01); vWF: The number of Ag gradually increased from 8 hours (P\u003c0.0.001); D? The content of D gradually increased from 16 hours (P\u003c0.05); PS: Until 48 hours, the content of Ag decreased significantly (P\u003c0.001); Is there a significant change in the content of III during AT? Pathological examination revealed that the lung, kidney, liver, spleen and other tissues were damaged to varying degrees, but there were no obvious venous thrombosis and bleeding characteristics.

　　Conclusion: The rat sepsis model has acute coagulation dysfunction, but is there no histopathological change caused by coagulation function?