动物造模,类风湿关节炎模型 z-bio 2021-12-23 294

　　Objective: To study the differences in symptoms and pathological characteristics between Wistar and Lewis rat rheumatoid arthritis (RA) models induced by bovine type II collagen?

　　Methods: The first intradermal injection and immunization with CFA mixture. On the 14th day, a mixture of type 2 bovine collagen (CII) and Freund's complete adjuvant (IFA) was injected to enhance immunity. We established Wistar and Lewis rat RA models , And confirmed the incidence of WistarA group and Lewis. Observe the rheumatoid arthritis group and measure the legs. At the 12th week, is there swelling and serum anti-CII antibody titer, foot morphology and histopathological examination? IL? 6? IL? 10. Measure the expression of IL?

　　Results: After the induction of collagen, Wistar and Lewis rats developed hindfoot swelling on the 10th and 14th days, and reached the peak on the 21st and 24th days. The serum anti-CII antibody titer was 3, which increased significantly after one week (P\ u003c0.01), the arthritis index (AI) increased significantly (P\u003c0.01); the modeling rate of the WistarA group was 80%, and the swelling of the feet was 5. In the flat phase, the modeling rate of the LewisA group was 100 %. During the first 7 weeks, the swelling of the feet subsided and entered a mild phase. At 12 weeks, both model groups suffered from severe cartilage erosion, synovial hyperplasia and inflammatory cell infiltration. Synovial angiogenesis and the formation of vascular nu, as well as the bone mineral density of the hind limbs and tibia are significantly reduced (P\u003c0.01), VEGF in the synovium? IL? 6 is IL? The expression of 17A was significantly reduced. Wistar? Compared with the RA group, it increased (P\u003c0.05, P\u003c0.01), and IL-10 expression was significantly reduced (P\u003c0.01) The increase in foot volume and thickness in the LuisA group lasted longer. In the RA group with higher AI, synovial angiogenesis and vascular pan formation were more obvious, and the expression of VEGF in the ankle synovium was significantly higher than that in the WistarA group (P

　　Conclusion: Both WistarA and LewisA models show symptoms such as joint swelling, deformity, decreased mobility and arthritis. , VEGF in the synovium of joints? IL? 6? IL? 17A, IL expression increased? The expression of 10 is reduced. Does this match the clinic? However, the expansion period of the WistarA model is very short. Although the LewisA model has a longer period of swelling, the joint damage is also more serious, and the formation of angiogenesis and vascular pan is more obvious. The WistarA model has a high expression of IL 17A, and the LewisA model has a high expression.