肾缺血再灌注损伤模型 z-bio 2021-12-21 336

　　Objective: To explore how to create a rat renal ischemia-reperfusion injury model that is less damaged, easy to operate and more stable.

　　Method: The SD male rats were randomly divided into normal group (C group), sham operation group (S group) and experimental group (IR group). The rats in the experimental group entered the bilateral retroperitoneal space through the dorsal fascia through the midline skin incision on the back, separated bilateral renal infarctions, clamped the bilateral renal infarctions with intact arteriole clamps for 50 minutes, and then released them. Perfusion: The rats in the sham operation group did not clamp the renal infarction, and the rest of the steps were the same as in the experimental group, only the rats in the normal group were anesthetized. Observe the patient's survival condition, changes in blood creatinine, urea nitrogen and kidney tissue structure 24 hours after surgery.

　　Results: Ir group skin incision (2 24 ± 0 27) cm, right lower back muscle membrane and muscle incision (1 36 ± 0 21) cm, left lower psoas muscle membrane and muscle incision (1) 36 36 ± 0 24 24) cm . It takes (3 30 ± 0 37) minutes from the skin incision to the kidney stem clamp. The success rate of the IR team's model establishment is 95%. Compared with the normal and sham operation groups, the blood creatinine and blood urea nitrogen levels in the IR group increased significantly (P\u003c0.01), and the renal tubular injury score increased significantly (P\u003c0). .05)

　　Conclusion: The renal ischemia-reperfusion injury model was established by the posterior peritoneal double renal tubule clamp method. The effect is stable, the success rate is high, the incision is small, the bleeding is small, and the animal is small.