动物造模,巴马小型猪高血压模型 z-bio 2021-10-19 273

　　Objective: How to establish a high-fat and high-salt diet of Pamani Buda hypertension model and explore its possible causes?

　　Method: 18 Bamamini male pigs were randomly divided into 3 groups: normal control group (NC)? Are there 6 animals in the high-fat (HF) group and the high-fat and high-salt (HFHS) group? Group C received regular diet feeding, and HF group and HFHS group received high-fat (HFHS) group feeding. The systolic blood pressure (SBP) and diastolic blood pressure (DBP) of mini-pigs on high-fat and high-salt diets were measured at the 8, 16, and 24 weeks of the modeling, respectively. Measure blood lipids, liver and kidney function, and plasma endothelin 1 (ET-) in the 24th week of modeling. 1) Take renin (renin), angiotensin II (AngII), aquaporin 2 (AQP-2), vasopressin (AVP) and vascular endothelial growth factor (VEGF) and other indicators, and the liver? Histopathological observation of the kidney?

　　Results: Compared with the NC group, the SBP and DBP of the HF group and the HFHS group increased significantly after 8 weeks, showing a trend of modeling and continuous increase. The HFHS group was higher than the HF group; at the same time, after 24 weeks of modeling, HF The body weight and liver and kidney indexes of minipigs in the HFHS group and HFHS group increased significantly (P\u003c0.05), and plasma TC, CREA and ET-1 levels also increased significantly (P\u003c0.05, P\u003c0.01). On the other hand, the BUN level of the HFHS group was significantly reduced (P\u003c0.05). , But renin? expensive? Me AQP-2? Significant increase in AVP content (P\u003c0.05, P\u003c0.01)? Oil red "O" staining results show HF liver and kidney lipid deposition. HFHS group and renal artery thickening and other pathological changes?

　　Conclusion: Mini-pig hypertension model can be established 8 weeks after induction of high-fat and high-salt diet. The etiology may be related to affecting renal function, activating the RAS system and AVP.