动物造模,肝门静脉注射肿瘤细胞 z-bio 2021-12-24 348

　　Objective: To establish a new animal model of advanced liver tumor by injecting tumor cells into the portal vein.

　　Method: Human hepatocellular carcinoma obtained by culturing MHCC97? H? Human colorectal cancer SW480? Human non-small cell lung cancer A549 and human triple negative breast cancer MDA? MB? 231 is the same as other cell lines. These malignant tumor cells are BALB/c nude mice liver hepatic portal vein. After 3-4 weeks of growth, PET/CT test: nuclear probe 18F is about 200μC? After 30-40 minutes, inject FDG into the tail vein and perform a PET/CT test in the BALB/c nude photos? Based on this, do you want to collect animals to obtain livers and take pictures to identify tumor lesions in the liver of BALB/c nude mice?

　　Result: MHCC97? H? SW480? A549 and MDA? MB? BALB/c nude mice with multiple diffuse tumor lesions of 231 cells formed in the liver of nude mice. The growth of tumor cells in the liver of nude mice can be detected by PET/CT. Is the PET/CT detection signal of diseased cells formed by SW480, MDA, MB, 231, A549, and MHCC97? Weak cells than H.?

　　Conclusion: We have successfully obtained a new animal model of advanced liver tumors, and laid a solid foundation for screening drugs related to advanced liver tumors.