动物造模,诺如病毒感染树鼩小动物模型 z-bio 2021-12-24 282

　　Objective: How to explore the feasibility of establishing a tree h virus infection model through tube feeding and attack?

　　Method: Two groups of six-month-old trees were given oral gavage to challenge GII.4 HuNoVs2010 and HuNoVs. The Sydney strain control group (5 animals in each group) was filled with the same amount of PBS. Signs and diarrhea were observed for 7 consecutive days after challenge. Stool was tested every 24 hours, and HuNoVsRNA was tested by RT. Was collected. -PCR, after killing the small white rabbits, observe the pathological changes from the small intestine tissue, HE staining, use immunohistochemistry to detect viral antigens, and identify human tissue blood group antigens (HBGA) and dendritic analysis of the saliva of Togarine rats?

　　Result: Treeshrew did not appear after being infected by HuNoV. Obvious symptoms of infection and diarrhea, HuNoVsNA was not detected in stool specimens, HuNoVs antigen was not detected in small intestine tissue, pathological changes were not obvious, antigens in HBGA tupai saliva were negative, were they?

　　Conclusion: The tree sh is not affected by HuNoV. The reason may be related to this. The non-secretion and expression of HBGA are related to general reproductive and reproductive conditions. Does the tree sh model of HuNoVs infection need further investigation?