动物造模,动脉粥样硬化 z-bio 2021-03-02 253

　　Objective: To explore an effective method to establish a dual-target mouse model of myocardial ischemia and atherosclerotic plaque angiogenesis?

　　Method: As the ApoE-/-10 mouse model group, use 10 C57BL/6J mice as the control group? Did the control group receive a normal diet, and did the model group receive a high-fat diet? The model group was fed a high-fat diet for 8 weeks, and was injected subcutaneously with 100 mg/kg isoproterenol for 2 consecutive days. , The control group was injected subcutaneously and so on. What is the amount of saline? After 4 weeks, serum total cholesterol (total cholesterol, total cholesterol), triglycerides (triglycerides, TG), low-density lipoprotein cholesterol (low-density lipoprotein cholesterol, LDL-C) and high-density lipoprotein cholesterol (High-density lipoprotein cholesterol, HDL-C) levels; HE staining to observe the pathological changes in different areas of the aorta and myocardium; CD31 immunohistochemical staining to detect the angiogenesis density in different parts of the aorta and myocardium?

　　Result: blood TC in the model group? The level of LDL-C was significantly higher than the control group (P\u003c0.05), but HDL-C was significantly lower than the control group (P\u003c0.05); HE staining was in the model aorta. It showed typical pathological changes of AS; 4 weeks after subcutaneous injection of isoproterenol, HE staining of myocardial tissue in the model group showed typical pathological changes of myocardial infarction; CD31 immunohistochemical staining showed that the aortic angiogenesis rate of the model group was significantly higher than that of the model group . In the control group (P\u003c0.05), the model group had the most abundant myocardial ischemia area, which was significantly higher than that of myocardial infarction. Normal myocardial tissue area (P\u003c0.05)?

　　Conclusion: ApoE-/- mice on a high-fat diet may cause myocardial infarction by subcutaneous injection of isoproterenol, and successfully established a dual-target mouse model of myocardial ischemia. Is this possible? Is it related to AS plaque angiogenesis?