气滞血瘀型慢性输卵管炎症大鼠模型,动物造模 z-bio 2021-03-02 287

　　Purpose: Will we explore the method of establishing a chronic salpingitis model with stagnation and hyperemia, study its feasibility, conduct a preliminary evaluation of the model, and lay the foundation for further experimental research?

　　Methods: Using unpredictable repetitive chronic stress stimulation (acoustic, optical, electrical, tail clip stimulation) to select SD rats, and by constructing a chronic renal tubular inflammation model, using bacterial renal tubular surgical injection for stasis and congestion pathology Observation and hemorheology are related to symptom evaluation and characterization. Preliminary assessment of establishing an animal model of chronic fallopian tube inflammation with index detection, stagnation and congestion?

　　Result: Compared with the model group, the total syndrome score of the complex in the model group was significantly increased (P\u003c0.01), and the fallopian tube was 80% in histological observation. % Have severe swelling of blood vessels, and 60% of patients have severe wall thickening. Pathological and morphological analysis showed that there was a lot of inflammatory cell infiltration. In the fallopian tube lumen of the composite model group, mucosal epithelial cells were shed and accompanied by a large number of inflammatory cell infiltration around the fallopian tube; is the agglutination index of the model group significantly increased (P\u003c0.01)?

　　Conclusion: Comprehensive evaluation and analysis found that the pathological changes of chronic qi stagnation and congestive salpingitis model rats are basically consistent with the pathological changes of congestive syndrome and chronic inflammation, and their symptoms are similar to human clinical symptoms. Is the modeling method feasible?