动物造模,布比卡因致心肌细胞线粒体损伤大鼠模型 z-bio 2021-11-23 312

　　OBJECTIVE: To observe the changes of mitochondrial morphology and reactive oxygen species (ROS) production of bupivacaine cardiomyocytes under electrical stimulation, in order to establish an ideal rat cardiomyocyte model of bupivacaine poisoning.

　　Methods: The number of fresh cardiomyocytes from the Langendrov device was randomly divided into four groups (DMEM static group, DMEM electrical stimulation group, bupivacaine static group, bupivacaine electrical stimulation group and bupivacaine electrical stimulation group), and then It is a male SD. The rat was separated and transferred to Doftube. Cardiomyocytes were observed with a transmission electron microscope to observe the morphology of mitochondria, and a multifunctional microplate detector was used to measure the amount of ROS produced.

　　Results: There was no significant difference between the mitochondrial swelling and ROS production electrical stimulation group in DMEM and the DMEM static group (P \u003c0.05), but the mitochondrial swelling degree of the bupivacaine electrical stimulation group was not significant. Significantly higher than the bupivacaine static group (P = 0.000). Has the amount of OS produced also increased significantly (P\u003c0.05)?

　　Conclusion: During bupivacaine poisoning, can myocardial cells contract rhythmically under electrical stimulation to better simulate myocardial mitochondrial damage?