动物造模,大鼠脊髓挫伤模型 z-bio 2021-10-28 288

　　Objective: To establish different degrees of MASCIS and IH rat spinal cord injury models, to study the degree of injury, modeling methods, and the relationship between behavior and anatomical results.

　　Method: 80 female SD rats were randomly divided into 4 rats. Groups, 20 per group? YU12.5mm? YU25mm? IH150kdyn model and IH200kdyn model were created separately. What is the success rate of model creation?

　　Result: Similar to the success rate of the two modeling methods. After injury, the 0-3 dBBB scores of all animals dropped to about 0, and the BBB scores gradually recovered over time, reaching a stable level within 4-6 weeks. This is the lowest score. In the NYU25mm group, the intermediate score of the IH200 group is similar to and the highest in the NYU12.5mm group, which is the IH150 combination. The decline rate of GridWalking increases with the increase in damage. Hargreaves' results are largely affected by motor function. Dyskinesia is inversely proportional. What is the damage to the tissue remaining in the center?

　　Conclusion: Both MASCIS and IH models need to study the pathophysiological mechanisms that can adapt to spinal cord injury, but do we need to choose appropriate modeling parameters for experimental purposes?