大鼠内脏高敏感模型,动物造模 z-bio 2021-10-27 641

　　Objective: How to establish an animal model of rat visceral hypersensitivity caused by plantar electric shock and evaluate its effectiveness and benefits?

　　Method: The female SD rats were randomly divided into normal control group (NC), plantar electric shock (FSS) and water avoidance group. Pressure (WAS) group? Group C was placed in a simulated pressure box for 1 hour a day without stimulation; group FSS was placed in a shock box for 1 hour a day and given electric shock stimulation, the stimulation voltage was 40 V, the stimulation frequency was 20 times/minute, each time was 1 second, total 5 minutes, and the WAS group is subjected to 1 hour of water avoidance pressure every day, and the modeling time is 10 days? Observe and record the general condition and defecation of the rats. Use ELISA method to detect abdominal wall contraction (AWR) and serum CRF to detect visceral sensitivity? ACTH? CORT? 5-HT concentration and colon tissue 5-HT concentration?

　　Results: (1) The visceral sensitivity of rats in the FSS group was significantly higher than that in the WAS and NC groups (P≥0.05). Is there any obvious pathological damage to the mucosa? (2) Serum CRF, ACTH, CORT, 5-HT concentration and colon tissue 5-FSS group rat HT concentration were significantly higher than WAS group and NC group (P\u003c0.05)? (3) The fecal volume of rats in the FSS group was significantly higher than that in the WAS and NC groups. (P\u003c0.05), and its stool water content was significantly higher than the NC group (P\u003c0.05), and its stool volume and stool water content are stable?

　　Conclusion: Does the foot shock modeling method better simulate rat visceral hypersensitivity than the avoiding water stress method?