动物造模,COPD-OSAHS 重叠综合征模型 z-bio 2021-10-14 305

　　Purpose: Will you establish a rat COPD-OSAHS overlap syndrome model to provide a feasible method and experimental platform for basic OS research?

　　Method: 14 SPF female 6-week-old SD rats were randomly divided into experimental groups. The control group and the experimental group (8 rats) smoke and intermittent hypoxia. The control group (6 rats) were exposed to fake cigarettes and normal oxygen. After 8 weeks, the viability of the experimental group rats was calculated, and the two groups were compared. of. What is the general condition of the rat? Blood gas analysis? Right ventricular hypertrophy index? The average number of alveoli? Average alveolar spacing? Pathological changes in lung tissue?

　　Result: The oxygen concentration-time curve of the intermittent hypoxia box shows that the oxygen concentration drops regularly and recovers normally, and we have successfully simulated the intermittent hypoxia environment. Did the experimental group survive with a 75% survival rate, and did all 6 rats in the control group survive? There are 6 surviving SD rats in this group. Compared with the control group, the experimental group is in a worse general condition. Is there acidosis? Hypoxia? Keep carbon dioxide? Right ventricular hypertrophy? Does it mean a significant increase in alveolar block? The average alveolar count was significantly reduced (P\u003c 0.05), and the difference was statistically significant; is the pathological observation of the lung tissue an inflammatory infiltration of the lung interstitium? Proliferation of lymphocytes in the bronchial wall? Smooth muscle hyperplasia of bronchial wall? Local destruction of smooth muscle of the tube wall? Goblet cell hyperplasia and emphysema?

　　Conclusion: The combination of smoking method and intermittent hypoxic exposure has successfully established a large mouse OS model. Is research needed to find more meaningful and objective model indicators?